rabbit anti mouse bfgf monoclonal antibody  (Bioss)

Journal: International Journal of Immunopathology and Pharmacology

Article Title: A monoclonal antibody against basic fibroblast growth factor attenuates cisplatin resistance in lung cancer by suppressing the epithelial-mesenchymal transition

doi: 10.1177/03946320221105134

Figure Lengend Snippet: The bFGFmAb and its characteristics. (a) Sodium dodecyl sulfate-polyacrylamide gel electrophoresis of the purified bFGFmAb (H, heavy chain; L, light chain). (b) Concentration of the purified bFGFmAb stock solution determined by bicinchoninic acid (BCA) standard assays. (c) Titer of the purified bFGFmAb stock solution tested by indirect ELISA. (d) The bFGFmAb inhibits the proliferation of A549 and A549/DDP cells. (e) Treatment with bFGF increases the proliferation in A549 and A549/DDP cells. bFGF, basic fibroblast growth factor; DDP, cisplatin; ELISA, enzyme-linked immunosorbent assay; mAb, monoclonal antibody; OD, optical density.

Article Snippet: After blocking with 5% blotto (skimmed milk) in Tris-buffered saline and Tween 20 for 1 h, the primary antibodies rabbit monoclonal anti-bFGF (CST, USA; 1:1000 dilution), mouse monoclonal anti-E-cadherin (CST, 1:1000 dilution), rabbit polyclonal anti-N-cadherin (Abcam, UK; 1:1000 dilution), rabbit polyclonal anti-LRP (Santa Cruz, USA, 1:200 dilution), and mouse monoclonal anti-β-actin (CST, USA, 1:1000 dilution) were added and incubated at 4°C overnight.

Techniques: Polyacrylamide Gel Electrophoresis, Purification, Concentration Assay, Indirect ELISA, Enzyme-linked Immunosorbent Assay

Journal: International Journal of Immunopathology and Pharmacology

Article Title: A monoclonal antibody against basic fibroblast growth factor attenuates cisplatin resistance in lung cancer by suppressing the epithelial-mesenchymal transition

doi: 10.1177/03946320221105134

Figure Lengend Snippet: Effects of bFGF and bFGFmAb on cisplatin resistance in non-small cell lung cancer.

Article Snippet: After blocking with 5% blotto (skimmed milk) in Tris-buffered saline and Tween 20 for 1 h, the primary antibodies rabbit monoclonal anti-bFGF (CST, USA; 1:1000 dilution), mouse monoclonal anti-E-cadherin (CST, 1:1000 dilution), rabbit polyclonal anti-N-cadherin (Abcam, UK; 1:1000 dilution), rabbit polyclonal anti-LRP (Santa Cruz, USA, 1:200 dilution), and mouse monoclonal anti-β-actin (CST, USA, 1:1000 dilution) were added and incubated at 4°C overnight.

Techniques:

Journal: International Journal of Immunopathology and Pharmacology

Article Title: A monoclonal antibody against basic fibroblast growth factor attenuates cisplatin resistance in lung cancer by suppressing the epithelial-mesenchymal transition

doi: 10.1177/03946320221105134

Figure Lengend Snippet: Effects of the bFGFmAb on the morphology of non-small cell lung cancer cells. EMT cell morphology is evident in both A549 and A549/DDP cells after treatment with bFGF. A549 cells become a long fusiform and spindle shaped phenotype, while A549/DDP cells transform to a long, thin and more irregular shape. Treatment of the cells with bFGFmAb attenuates/reverses the bFGF-induced EMT transformation. bFGF, basic fibroblast growth factor; DDP, cisplatin; EMT, epithelial-to-mesenchymal transition; mAb, monoclonal antibody.

Article Snippet: After blocking with 5% blotto (skimmed milk) in Tris-buffered saline and Tween 20 for 1 h, the primary antibodies rabbit monoclonal anti-bFGF (CST, USA; 1:1000 dilution), mouse monoclonal anti-E-cadherin (CST, 1:1000 dilution), rabbit polyclonal anti-N-cadherin (Abcam, UK; 1:1000 dilution), rabbit polyclonal anti-LRP (Santa Cruz, USA, 1:200 dilution), and mouse monoclonal anti-β-actin (CST, USA, 1:1000 dilution) were added and incubated at 4°C overnight.

Techniques: Transformation Assay

Journal: International Journal of Immunopathology and Pharmacology

Article Title: A monoclonal antibody against basic fibroblast growth factor attenuates cisplatin resistance in lung cancer by suppressing the epithelial-mesenchymal transition

doi: 10.1177/03946320221105134

Figure Lengend Snippet: The bFGFmAb regulates markers for EMT and suppresses LRP expression. Treatment with bFGFmAb leads a significantly decrease in expression of bFGF, N-cadherin, and LRP, and a significantly increases in the expression of E-cadherin in both A549 and A549/DDP cells. N-cadherin and E-cadherin are EMT markers. * p < 0.05. bFGF, basic fibroblast growth factor; DDP, cisplatin; EMT, epithelial-to-mesenchymal transition; LRP, lung resistance protein; mAb, monoclonal antibody.

Article Snippet: After blocking with 5% blotto (skimmed milk) in Tris-buffered saline and Tween 20 for 1 h, the primary antibodies rabbit monoclonal anti-bFGF (CST, USA; 1:1000 dilution), mouse monoclonal anti-E-cadherin (CST, 1:1000 dilution), rabbit polyclonal anti-N-cadherin (Abcam, UK; 1:1000 dilution), rabbit polyclonal anti-LRP (Santa Cruz, USA, 1:200 dilution), and mouse monoclonal anti-β-actin (CST, USA, 1:1000 dilution) were added and incubated at 4°C overnight.

Techniques: Expressing

Journal: Regenerative Therapy

Article Title: Recombinant canine basic fibroblast growth factor-induced differentiation of canine bone marrow mesenchymal stem cells into voltage- and glutamate-responsive neuron-like cells

doi: 10.1016/j.reth.2020.07.005

Figure Lengend Snippet: The expression of rc-bFGF mRNA in the purification process of rc-bFGF by the cell-free protein synthesis system. Lane 1–6: the results of RT-PCR in reaction solution after synthesis of canine bFGF. Lane 7-12: the results of RT-PCR in reaction solution without synthesis of canine bFGF (negative control). Lane 13–18: the results of PCR in the pET28a plasmid for synthesis by E. coli (positive control).

Article Snippet: The membranes were incubated with the anti-rabbit monoclonal bFGF antibody (Cell Signaling Technology Inc., Danvers, MA, U.S.A.).

Techniques: Expressing, Purification, Reverse Transcription Polymerase Chain Reaction, Negative Control, Plasmid Preparation, Positive Control

Journal: Regenerative Therapy

Article Title: Recombinant canine basic fibroblast growth factor-induced differentiation of canine bone marrow mesenchymal stem cells into voltage- and glutamate-responsive neuron-like cells

doi: 10.1016/j.reth.2020.07.005

Figure Lengend Snippet: Western blotting of purified protein. Band formation was observed at almost same position as rc-bFGF synthesized using E. coli (arrows). Lane 1: reaction solution after synthesis of canine bFGF. Lane 2: reaction solution without synthesis of canine bFGF (negative control). Lane 3: rc-bFGF synthesized using E. coli, Lane 4: commercially available rh-bFGF (positive control).

Article Snippet: The membranes were incubated with the anti-rabbit monoclonal bFGF antibody (Cell Signaling Technology Inc., Danvers, MA, U.S.A.).

Techniques: Western Blot, Purification, Synthesized, Negative Control, Positive Control

Journal: Regenerative Therapy

Article Title: Recombinant canine basic fibroblast growth factor-induced differentiation of canine bone marrow mesenchymal stem cells into voltage- and glutamate-responsive neuron-like cells

doi: 10.1016/j.reth.2020.07.005

Figure Lengend Snippet: Phosphorylation of ERK in HEK293 cells after stimulation by purified protein. pERK expression was observed after stimulation of HEK293 cells by our purified protein, as it was after stimulation by commercially available rh-bFGF. Lane 1: extract from HEK293 cells after no stimulation of rc-bFGF or rh-bFGF (negative control). Lane 2: extract from HEK293 cells after stimulation by 5 ng/mL of rc-bFGF. Lane 3: extract from HEK293 cells after stimulation by 15 ng/mL of rc-bFGF. Lane 4: rc-bFGF synthesized solution only. Lane 5: extract from HEK293 cells after stimulation by 5 ng/mL of commercially available rh-bFGF (positive control). Lane 6: blotting buffer.

Article Snippet: The membranes were incubated with the anti-rabbit monoclonal bFGF antibody (Cell Signaling Technology Inc., Danvers, MA, U.S.A.).

Techniques: Phospho-proteomics, Purification, Expressing, Negative Control, Synthesized, Positive Control

Journal: Regenerative Therapy

Article Title: Recombinant canine basic fibroblast growth factor-induced differentiation of canine bone marrow mesenchymal stem cells into voltage- and glutamate-responsive neuron-like cells

doi: 10.1016/j.reth.2020.07.005

Figure Lengend Snippet: The morphologies of canine BMSCs at 10 days of neuronal induction. Canine BMSCs were changed to a neuron-like morphology after neuronal induction in the rh-bFGF and the rc-bFGF groups (arrows). A: control group, B: undifferentiated group, C: rh-bFGF group, and D: rc-bFGF group.

Article Snippet: The membranes were incubated with the anti-rabbit monoclonal bFGF antibody (Cell Signaling Technology Inc., Danvers, MA, U.S.A.).

Techniques: Control

Journal: Regenerative Therapy

Article Title: Recombinant canine basic fibroblast growth factor-induced differentiation of canine bone marrow mesenchymal stem cells into voltage- and glutamate-responsive neuron-like cells

doi: 10.1016/j.reth.2020.07.005

Figure Lengend Snippet: The percentage of canine BMSCs that had changed to a neuron-like morphology at 10 days of neuronal induction. Error bars show mean ± standard error (n = 6 in each group). Asterisks indicate statistical difference among the groups ( P < 0.05). Control; control group, Undifferent; undifferentiated group, rh-bFGF; rh-bFGF group, rc-bFGF; rc-bFGF group.

Article Snippet: The membranes were incubated with the anti-rabbit monoclonal bFGF antibody (Cell Signaling Technology Inc., Danvers, MA, U.S.A.).

Techniques: Control

Journal: Regenerative Therapy

Article Title: Recombinant canine basic fibroblast growth factor-induced differentiation of canine bone marrow mesenchymal stem cells into voltage- and glutamate-responsive neuron-like cells

doi: 10.1016/j.reth.2020.07.005

Figure Lengend Snippet: The mRNA expression of neuronal markers pre- and post-neuronal induction. A: NEFL , B: NEFH , C: MAP2 , and D: TUBB3 . Error bars show mean ± standard error (n = 6 in each group). Asterisks indicate statistical difference between pre- and post-neuronal induction ( P < 0.05). Control; control group, Undifferent; undifferentiated group, rh-bFGF; rh-bFGF group, rc-bFGF; rc-bFGF group.

Article Snippet: The membranes were incubated with the anti-rabbit monoclonal bFGF antibody (Cell Signaling Technology Inc., Danvers, MA, U.S.A.).

Techniques: Expressing, Control

Journal: Regenerative Therapy

Article Title: Recombinant canine basic fibroblast growth factor-induced differentiation of canine bone marrow mesenchymal stem cells into voltage- and glutamate-responsive neuron-like cells

doi: 10.1016/j.reth.2020.07.005

Figure Lengend Snippet: Relative fluorescence intensity of intracellular Ca 2+ concentration after the stimulation by 50 mM KCl (A, B, C, D) or 100 μ M l -glutamate (E, F, G, H). A and E: Control group, B and F: Undifferentiated group, C and G: rh-bFGF group, D and H: rc-bFGF group.

Article Snippet: The membranes were incubated with the anti-rabbit monoclonal bFGF antibody (Cell Signaling Technology Inc., Danvers, MA, U.S.A.).

Techniques: Fluorescence, Concentration Assay, Control